Light-Sheet Microscopy for Live Imaging
نویسندگان
چکیده
منابع مشابه
Light Sheet Module Applied in 3d Live Cell Microscopy
Innovative fiber-coupled add-ons for light sheet-based fluorescence microscopy (LSFM) with commercial inverted microscopes were described previously [1]. The light sheet is generated either by an achromatic cylindrical lens or by a spherical mirror with astigmatic distortion. Presently the “lens system” is further validated and applied to various samples including 3D multicellular spheroids and...
متن کاملLive imaging of nervous system development and function using light-sheet microscopy.
In vivo imaging applications typically require carefully balancing conflicting parameters. Often it is necessary to achieve high imaging speed, low photo-bleaching, and photo-toxicity, good three-dimensional resolution, high signal-to-noise ratio, and excellent physical coverage at the same time. Light-sheet microscopy provides good performance in all of these categories, and is thus emerging a...
متن کاملThree-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM).
We describe a method for the three-dimensional live imaging of filamentous fungi with light sheet-based fluorescence microscopy (LSFM). LSFM provides completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model Podospora anserina. The protocol explains the mounting ...
متن کاملLight-sheet optimization for microscopy
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM)...
متن کاملHyperspectral light sheet microscopy
To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varyin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Review of Laser Engineering
سال: 2013
ISSN: 0387-0200,1349-6603
DOI: 10.2184/lsj.41.2_103